Thursday, March 28, 2019

Chemical Imaging for Biomedicine: The Next Frontier of Light Microscopy


Life Science Microscopy 
 The latest life science microscopy news from AZoNetwork 
 #ALT#Chemical Imaging for Biomedicine: The Next Frontier of Light Microscopy
Metabolites and drugs are hard to visualize because they are mostly small molecules, do not emit fluorescence and cannot be labeled with the large fluorophores. However, they are very important in biomedicine because a lot of diseases are related to abnormalities in metabolism. This article describes the development of new imaging techniques to overcome these challenges: Bioorthogonal Chemical Imaging for small metabolites and Super Multi-Color Imaging.
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   Types of Scanning Probe MicroscopyTypes of Scanning Probe Microscopy
 
There are several different types of scanning probe microscopes, the most prominent of which are atomic force microscopy (AFM) and scanning tunneling microscopy (STM). There are also many other types, which are listed at the end of this article.
 
   Avoiding Cell Death in Fluorescence MicroscopyAvoiding Cell Death in Fluorescence Microscopy
 
Fluorescence microscopy is a common laboratory tool used to investigate the physiology of different cells. Specific wavelengths of light are shone onto cells in a sample, where the light is absorbed by molecules called fluorophores.
 
 Microsphere-Assisted Microscopy: Advantages and Limitations
 
Microsphere-Assisted Microscopy: Advantages and LimitationsTransparent microspheres can generate an image with a super-resolution of 50 nanometers. This technique provides several advantages over conventional imaging and confocal microscopy.
 
 
 E-FRET Microscopy for Live Cell Imaging
 
E-FRET Microscopy for Live Cell ImagingE-FRET microscopy recently became a very popular intensity-based Fluorescence Resonance Energy Transfer (FRET) method for cell quantification. This technique reduces the photobleaching observed during the process of FRET.
 
 
 Applications of Cryo-Electron Microscopy
 
Applications of Cryo-Electron MicroscopyHigher resolution of any image is achieved by the wavelength of the radiation used for imaging. A typical electron microscopy uses a beam of electrons in the wavelength of ~ 0.02å for operation at 300kV.
 
 
 Portable mini microscope could improve scientists' ability to study brain disease in living mice
 
Portable mini microscope could improve scientists' ability to study brain disease in living miceWorking with mice, a team of Johns Hopkins Medicine researchers has developed a relatively inexpensive, portable mini microscope that could improve scientists' ability to image the effects of cancer, stroke, Alzheimer's disease and other conditions in the brains of living and active mice over time.
 

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