Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation | BMC Physiology | Full Text

Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation | BMC Physiology | Full Text

BMC Physiology

Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation

• Inka Gallitz,
• Niklas Lofruthe,
• Lisa Traeger,
• Nicole Bäumer,
• Verena Hoerr,
• Cornelius Faber,
• Tanja Kuhlmann,
• Carsten Müller-Tidow and
• Andrea U. SteinbickerEmail author

BMC PhysiologyBMC series – open, inclusive and trusted201818:3

https://doi.org/10.1186/s12899-018-0037-z

©  The Author(s). 2018

• Received: 3 March 2017
• Accepted: 13 February 2018
• Published: 27 February 2018

Abstract

Background

Inflammatory stimuli induce the hepatic iron regulatory hormone hepcidin, which contributes to anaemia of inflammation (AI). Hepcidin expression is regulated by the bone morphogenetic protein (BMP) and the interleukin-6 (IL-6) signalling pathways. Prior results indicate that the BMP type I receptor ALK3 is mainly involved in the acute inflammatory hepcidin induction four and 72 h after IL-6 administration. In this study, the role of ALK3 in a chronic model of inflammation was investigated. The intact, heat-killed bacterium Brucella abortus(BA) was used to analyse its effect on the development of inflammation and hypoferremia in mice with hepatocyte-specific Alk3-deficiency (Alk3fl/fl; Alb-Cre) compared to control (Alk3fl/fl) mice.

Results

An iron restricted diet prevented development of the iron overload phenotype in mice with hepatocyte-specific Alk3 deficiency. Regular diet leads to iron overload and increased haemoglobin levels in these mice, which protects from the development of AI per se. Fourteen days after BA injection Alk3fl/fl; Alb-Cre mice presented milder anaemia (Hb 16.7 g/dl to 11.6 g/dl) compared to Alk3fl/fl control mice (Hb 14.9 g/dl to 8.6 g/dl). BA injection led to an intact inflammatory response in all groups of mice. In Alk3fl/fl; Alb-Cre mice, SMAD1/5/8 phosphorylation was reduced after BA as well as after infection with Staphylococcus aureus. The reduction of the SMAD1/5/8 signalling pathway due to hepatocyte-specific Alk3deficiency partly suppressed the induction of STAT3 signalling.

Conclusion

The results reveal in vivo, that 1) hepatocyte-specific Alk3 deficiency partly protects from AI, 2) the development of hypoferremia is partly dependent on ALK3, and 3) the ALK3/BMP/hepcidin axis may serve as a possible therapeutic target to attenuate AI.

Keywords

• Bone Morphogenetic Protein (BMP) type I receptor
• Inflammation
• Iron
• Hepcidin
• Liver